The nitrate reductase of chlorella: species or strain differences.
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چکیده
منابع مشابه
Partial Purification of the NADH-Nitrate Reductase Complex from Chlorella pyrenoidosa.
The nitrate reductase complex from Chlorella pyrenoidosa has been purified by a procedure which includes as main steps, ammonium sulfate fractionation, polyethylene glycol treatment, and DEAE-cellulose chromatography. The Michaelis constants for NADH, FAD, and NO(3) (-) in the NADH-nitrate reductase assay are 10 mum, 2.6 mum, and 0.23 mm, respectively. Heat treatment exerts varying effects on t...
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Incubation of the complex metalloflavoprotein, assimilatory nitrate reductase with N-ethylmaleimide, or a spin-labeled analog, 4-maleimido-2,2,6,6-tetramethylpiperidinooxyl, resulted in a time-dependent inactivation of NADH:nitrate reductase and NADH: cytochrome-c reductase activity with no effect on reduced methyl viologen:nitrate reductase activity. Inactivation of the enzyme, which could be ...
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After x-ray irradiation, 13 mutants of Chlorella sorokiniana incapable of using NO(3) (-) as N source were isolated using a pinpoint method. Using immunoprecipitation and Western blot assays, no nitrate reductase was found in five strains while in eight mutants the enzyme was detected. The latter strains contained different patterns of nitrate reductase partial reactions. All isolates were of t...
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Nitrate is the predominant form of inorganic nitrogen utilized by higher plants, fungi and algae and its assimilation forms the major pathway for production of biologically useful nitrogen. Chlorella is a unicellular eukaryotic green alga, which offers a potentially useful system for the expression of hetrologous proteins. In the present study the microalgal samples were isolated from stagnant ...
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Molybdenum is absolutely required for the nitrate-reducing activity of the nicotinamide adenine dinucleotide nitrate reductase complex isolated from Chlorella fusca. The whole enzyme nicotinamide adenine dinucleotide nitrate reductase is formed by cells grown in the absence of added molybdate, but only its first activity (nicotinamide adenine dinucleotide diaphorase) is functional. The second a...
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ورودعنوان ژورنال:
- Plant physiology
دوره 49 6 شماره
صفحات -
تاریخ انتشار 1972